In an organization where there are individuals who consider their interests, is it possible to have individuals who would strive for the benefit of others without regard to any interests? Studies conducted from the past to the present day indicate that political behaviors affect personal attitudes and behaviors. This study aims to investigate the indirect effect of political behavior perception on prosocial motivation through organizational trust. Data were obtained voluntarily from 225 full-time employees of a company operating in the public service sector by using survey method. SPSS and AMOS programs were used to analyze the data. It was found that organizational trust mediated the relationship between political behavior perception and prosocial motivation. Besides, it was determined that political behavior perception and organizational trust were negatively related and organizational trust and prosocial motivation were positively related. This study will make a significant contribution to the literature as it reveals that prosocial motivation is based on the norms of reciprocity within the context of social exchange theory, and will contribute to the studies discussing that argue that there must be several precursors in the emergence of prosocial motivation in employees.