This study is a cross-sectional area study conducted in a public hospital to reveal the relationship between mobbing and motivation on health employees. The population of the study consisted of 1076 employees and applied to 222 hospital employees. The data gathered were analyzed by the SPSS 22.0 program. The majority of the participants were under 45 years old and female. According to the results of the study show that the health services in the hospital are given by health care professionals who are female. Besides, about 1/3 of the participants were single. 90.1% of the surveyed employees had no management experience before. When education level examined, the highest rate belongs to the bachelor’s group. Approximately 1/3 of the participants were nurses/midwives. About half of the participants were in the daytime; most of them work over 40 hours; the vast majority of them are under 10 years. The 2/3 of the participants was affiliated to the Ministry of Health. As a result of the correlation analysis, there was no statistically significant relationship between mobbing and motivation tools (p˃0.05). According to regression analysis, mobbing affects motivation tools (F = 3,954, R² = 0,018; F = 9,351, R² = 0,041).